Ultrasensitive Rat Insulin (10-1251-01)

Mercodia Ultrasensitive Rat Insulin ELISA provides a method for the quantitative determination of low levels of insulin in rat samples. Kit format: 1 x 96 wells Measurement Range: 0.025 – 1.5µg/L Very low detection limit – 0.02 µg/L High specificity – Insignificant cross-reactivity to C-peptide and proinsulin No interference from complex sample matrices Test Characteristics Samples: Serum, EDTA plasma and cell culture medium. Sample volume: 25 µL Assay range: 0.02 – 1.0 μg/L Detection limit: ≤ 0.02 µg/L Incubation: 2 h+ 30 min Specicifity The ELISA kit measures rat insulin to 100%. Mouse insulin 75 % Rat proinsulin I 8 % Rat proinsulin II 51 % Mouse proinsulin I 31 % Mouse proinsulin II 51 % Mouse c-peptide I n.d. Mouse c-peptide II n.d. Rat c-peptide I n.d. Rat c-peptide II n.d. Human insulin 167 % Human proinsulin 75 % Human C-peptide n.d. Insulin lispro (Humalog®) 167 %

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