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The Mercodia Ultrasensitive C-peptide ELISA is a high quality immunoassay for specific quantification of human C-peptide in serum, plasma, cell culture media or urine. Kit format: 1 x 96 wells Measurement range: 5 – 280 pmol/L (0.015 – 0.85 ng/mL) High sensitivity, detetction limit < 2.5 pmol/L (0.0076 µg/L) No or minimal cross-reactivity to insulin or proinsulin Calibrated against International Reference Reagent IRR84/510 for human C-peptide. CE/IVD labeled on select markets Test Characteristics Samples: Serum, EDTA and heparin plasma as well as urine. Sample volume: 50 µL Assay range: 5 – 280 pmol/L (0.015 – 0.85 ng/mL) Detection limit: 2.5 pmol/L (0.0076 µg/L) Incubation: 1 h + 1 h + 30 min Detection method: absorbance Specificity The following cross-reactions have been tested: Insulin < 0.0006 % Proinsulin 5 % Proinsulin (Des 31-32) 3 % Proinsulin (Split 32-33) 2 % Proinsulin (Des 64-65) 74 % Proinsulin (Split 65-66) 10 %

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