Ultrasensitive Insulin ELISA (10-1132-01)

A high quality enzyme immunoassay for specific quantification of human insulin in serum or plasma. Kit format: 1 x 96 wells Measurement range: 0.15 – 20 mU/L (0.0065 – 0.87 µg/L) Calibrated against 1st International Reference Preparation 66/304 for human insulin No cross-reactivity to C-peptide or Proinsulin des(31-32) or split (32-33) Insignificant or no cross-reactivity to insulin analogs CE/IVD labeled on select markets Test Characteristics Samples: Serum, plasma Sample volume: 25 µL Assay range: 0.15 – 20 mU/L Detection limit: ≤ 0.15 mU/L (0.0065 µg/L) Incubation: 1 h + 30 min Detection method: absorbance Specificity The following cross-reactions have been tested: C-peptide <0.01 % Proinsulin <0.01 % Proinsulin des (31-32) <0.5 % Proinsulin split (32-33) <0.5 % Proinsulin des (64-65) 98 % Proinsulin split (65-66) 56 % Insulin aspart 3 % Insulin detemir n.d. Insulin glargine 12 % Insulin glulisine n.d. Insulin lispro n.d. IGF-I <0.02 % IGF-II <0.02 % Rat insulin 0.7 % Mouse insulin 0.3 % Porcine insulin 93 % Ovine insulin 48 % Bovine insulin 31 % n.d.=not detected

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