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ELISA kit for quantitative determination of rat and mouse proinsulin in serum, plasma, cell culture media or tissue homogenate. Kit format: 1 x 96 wells Measurement range: 3.5 – 200 pmol/L Specific for rat and mouse proinsulin I and II No cross reactivity to insulin or C-peptide Fast and easy-to-use Test Characteristics Samples: Serum, plasma, cell culture medium, tissue homogenate Sample volume: 25 µL Assay range: 3.5 – 200 pmol/L Detection limit: ≤ 3 pmol/L Incubation: 120 + 30 min Specificity Rat proinsulin I 100% Rat proinsulin II 145 % Rat insulin n.d. Rat C-peptide n.d. Mouse insulin n.d. Mouse C-peptide n.d. Mouse proinsulin I 234 % Mouse proinsulin II 100 % Human insulin n.d. Human C-peptide n.d. Human proinsulin 0.5 %

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