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Immunoassay for quantitative determination of rat C-peptide in serum, plasma or cell culture medium. Kit format: 1 x 96 wells Measurement range: 100 – 4000 pmol/L (0.33 – 13 µg/L) Low sample volume – 10μL Measures both Rat C-peptide I and II Minimal cross-reactivity to human or mouse insulin, C-peptide or proinsulin Test Characteristics Samples: serum,EDTA plasma or cell culture media Sample volume: 10 µL Assay range: 100 – 4000 pmol/L (0.33 – 13 µg/L) Detection limit: ≤ 100 pmol/L (0.33 μg/L) Incubation: 1h + 1h + 15 min Detection method: absorbance Specificity The ELISA kit measures both Rat C-peptide I and Rat C-peptide II. The following cross-reactions have been tested: Rat insulin n.d. Rat proinsulin 4.6 % Mouse C-peptide 6 % Porcine C-peptide n.d. Human C-peptide n.d.

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