Proinsulin ELISA (10-1118-01)

Mercodia Proinsulin ELISA provides a method for the quantitative determination of human proinsulin in serum or plasma. Kit format: 1 x 96 wells Measurement range: 3.3 – 130 pmol/L (0.03 – 1.18 µg/L) Measures total proinsulin Calibrated against International Reference Reagent IRR 84/611 for human proinsulin Highly specific with no cross-reactivity to human insulin or C-peptide Test Characteristics Samples: Serum and plasma Sample volume: 50 µL Assay range: 3.3 – 130 pmol/L Detection limit: 1.7 pmol/L Incubation: 1 h + 1 h + 15 min Detection method: absorbance Specificity The assay is highly specific with no cross-reactivity to human insulin or C-peptide. The following cross-reactions have been tested: Insulin < 0.03 % C-peptide < 0.006 % Proinsulin (Des 31-32) 95 % Proinsulin (Split 32-33) 95 % Proinsulin (Des 64-65) 84 % Proinsulin (Split 65-66) 90 % Porcine Proinsulin 4 % Mouse Proinsulin I 3 % Mouse Proinsulin II 16 % Rat Proinsulin I 13 % Rat Proinsulin II 10 %

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