Iso-Insulin ELISA (10-1128-01)

Mercodia Iso-Insulin ELISA provides a method for the quantitative determination of total insulin. Kit format: 1 x 96 wells Measurement range: 3 – 100 mU/L (0.13 – 4.35 µg/L) Measures total insulin Cross-reactivity with most insulin analogs Calibrated against 1st International Reference Preparation 66/304 for human insulin. CE/IVD labeled on select markets Test Characteristics Samples: Serum, plasma, cell culture medium Sample volume: 25 µL Assay range: 3 – 100 mU/L Detection limit: 2 mU/L (0.086 µg/L) Incubation: 1 h + 15 min Detection method: absorbance Specificity The following cross-reactions have been tested: Insulin 100 % C-peptide < 0.1 % Proinsulin 54 % Proinsulin des (31-32) 58 % Proinsulin split (32-33) 56 % Proinsulin des (64-65) 66 % Proinsulin split (65-66) 78 % Insulin lispro 112 % Insulin aspart 100 % Insulin determir 28 % Insulin glargine 58 % Insulin glulisine 123 % IGF-I < 0.02 % IGF-II < 0.02 % Rat insulin 71 % Mouse insulin 49 % Porcine insulin 76 % Ovine insulin 131 % Bovine insulin 58 %

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