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Mercodia High Range Rat Insulin ELISA provides a method for the quantitative determination of high levels of insulin in rat samples. Kit format: 1 x 96 wells Measurement range: 3 – 150 µg/L No sample dilution for sample with high insulin concentrations Low sample volume – 10 µL Results within 2.5 hours Test Characteristics Samples: Serum, EDTA plasma and cell culture medium. Sample volume: 10 µL Assay range: 3 – 150 μg/L Detection limit: ≤ 1.5 µg/L Incubation: 2 h + 15 min Specificity The ELISA kit measures rat insulin to 100%. Mouse insulin 75% Rat C-peptide I n.d. Rat C-peptide II n.d. Mouse C-peptide I n.d. Mouse C-peptide II n.d. Rat Proinsulin I 8% Rat Proinsulin II 51% Mouse Proinsulin I 33% Mouse Proinsulin II 51% Human C-peptide n.d. Human insulin 167% Human proinsulin 75% Insulin lispro (Humalog® Eli Lilly) 167%

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