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Mercodia Glucagon ELISA – 10 μL is a highly specific and sensitive immunoassay for measuring glucagon in serum, EDTA plasma, and cell culture media samples from rat, mouse, and non-human primates. Kit format: 1 x 96 wells Measurement range: 2-180 pmol/L (7 – 627 pg/mL) Low sample volume – 10 µL No purification of samples Sample types: Serum, EDTA Plasma, and cell-culture medium Measurement range: 2 – 180 pmol/L (7 – 627 pg/mL) Detection limit: 1.5 pmol/L (5.23 pg/mL) Little or no cross-reactivity to oxyntomodulin, glicentin, mini-glucagon, GLP-1, GLP-2 and GRPP Highly appreciated and often cited assay Test Characteristics Samples: Serum, EDTA plasma and cell culture medium. Sample volume: 10 µL Assay range: 2-180 pmol/L (7 – 627 pg/mL) Detection limit: 1.5 pmol/L Incubation: 18-22 h (overnight) + 15 min Detection method: absorbance Specificity Mini-glucagon, n.d. Glicentin, human 1.0 % Glicentin, mouse 7.0 % Glicentin, rat 4.0 % Oxyntomodulin, human/rat/mouse 2.0 % Oxyntomodulin, bovine/canine/porcine n.d. GLP-1 (7-36), n.d. GLP-1 (9-36), n.d. GLP-2, n.d.

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