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The Mercodia Glicentin ELISA is a high quality immunoassay for the quantification of human glicentin in serum, plasma and cell culture media. Kit format: 1×96 wells Measurement range: 3 – 300 pmol/L (24 – 2400 pg/mL) Highly specific, sensitive and rapid method for determination of glicentin No cross-reactivity to circulating proglucagon derived gut peptides Assay buffers and calibrators have been optimized to prevent degradation and aggregation of glicentin Test Characteristics Samples: Serum, EDTA plasma and cell culture media Sample volume: 25 µL Assay range: 3 – 300 pmol/L (24-2400 pg/mL) Detection limit: 3 pmol/L (24 pg/mL) Incubation: 1 h + 1 h + 15 min Detection method: absorbance Specificity The following cross-reactions have been tested: Glucagon N.D. Oxyntomodulin N.D. Mini-glucagon N.D. GLP-1 N.D GLP-2 N.D. Rat glicentin 0.9 % Mouse glicentin N.D

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