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Rapidly Standardise & Scale Your ChIP Workflows

Rapidly Standardise & Scale Your ChIP Workflows
30/01/2019

Chromatrap® has entered into a partnership with Covaris to offer a highly scalable and simple ChIP workflow enabling researchers to easily isolate protein-DNA complexes for downstream applications, such as ChIP-Seq, ChIP-qPCR, Next-generation sequencing (NGS), and Mass Spectrometry (MS).

Preparing samples for Chromatin Immunoprecipitation (ChIP) has traditionally been a multi-step, time-consuming, and error-prone workflow. Pairing the bead-free Chromatrap immunoprecipitation system with the Covaris truChIP® and AFA® sample preparation technologies enables investigators to rapidly profile protein-DNA interactions of interest. By combining the two technologies, researchers now have access to a compatible and optimised workflow that increases productivity and allows the user to gain better results with higher quality samples.

Chromatrap, the revolutionary automation-compatible spin column-based approach eliminates many of the challenges encountered when working with magnetic beads. This rapid centrifugation-based protocol requires only 3 simple washes using individual spin columns or 96-well plates and can be completed in a single day. In contrast, typical bead-based workflows take around 10 hours with over 10 wash steps and incubations.

Using the Covaris Adaptive Focused Acoustics® (AFA®) technology, researchers can easily isolate and reproducibly shear soluble chromatin without disrupting the protein-DNA interactions for immunoprecipitation with bead-free Chromatrap Protein A and G ChIP kits.

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