search

ChromaTrap Receives Positive Commentary in Cited Research

ChromaTrap Receives Positive Commentary in Cited Research
20/03/2014

Porvair Sciences reports on positive commentary by researchers in published papers citing how using the fast, sensitive ChromaTrap™ Chromatin Immunoprecipitation (ChIP) technology has benefited their research.

In a paper by C.R Williams et al (Am J Physiol Cell Physiol 303: C213–C223, 2012) - Chromatrap was used to assess the occupation of NFkB p65 onto the Nox4 promoter in human aortic endothelial cells which had been treated with normal and high glucose. ChIP data using Chromatrap pro A spin columns demonstrated that in high glucose concentrations NfkB binding to the Nox4 promoter was increased when compared to normal glucose concentrations and rosiglitazone attenuated NfkB binding to the promoter. Their findings illustrated that NFkB/p65-Nox4 axis as an important target of rosigliatzone in vascular endothelial cells following hyperglycemia.

In a paper by C.Sullivan et al (PLOS ONE, Dec 2012, 7 (12), pp 7-13) Chromatrap was used to look at the association of FOXM1 transcription factor onto the CDC25A gene in a human (U205) osteosarcoma cell line and a CWR22rv prostate cancer cell line. Binding activity of FOXM1 onto CDC25A was assessed using Chromatrap Pro A columns which showed a significant enrichment of FOXM1 onto the CDC25A gene when compared to negative IgG in both cell lines. The study supports the hypothesis and reports a novel mechanism by which CDC25A is a direct target gene of the FOXM1 transcription factor. Both papers make use of the higher speed of the Chromatrap ChIP protocol (less than 5 hours), the ease of use of a solid-state assay and the excellent DNA pull down and high signal to noise ratio driven by the kit’s very low non-specific binding.

Launched worldwide in 2012 - The Chromatrap® solid-state ChIP technology has been shown in many projects to be more efficient than conventional bead-based methods. This is because the solid phase porous polymer, functionalized with either protein A or G, provides a greater surface area for chromatin antibody binding with very low non-specific binding. In addition, it uses a spin column approach, offering significant speed, process and carry-over advantages over sepharose or magnetic beads. DNA pull down with Chromatrap® is up to 25 times more than conventional methods, whilst the signal to noise ratio for DNA enrichment is 2 to 3 times better, even with low chromatin samples between 50ng to 3000ng per immunoprecipitation.

For further information about the Chromatrap® ChIP assay technology and related ChIP products please visit www.chromatrap.com or contact Porvair Sciences on +44-1978-666239 or int.sales@porvairsciences.com.

Established in 1992, Porvair Sciences Ltd. has developed internationally recognised expertise in microplate technology and manufacturing serving applications in Drug Discovery, Combinatorial Chemistry, Solid Phase Extraction, Protein Purification, High Throughput Screening, Environmental Analysis, Proteomics and Genomics. Porvair Sciences Ltd. is a wholly owned subsidiary of Porvair plc.

Enquiry Form



More News

Zero Crosstalk Luminescence Assay Plate

Zero Crosstalk Luminescence Assay Plate

Constructed in ultra-pure grade polystyrene, the opaque white Krystal 2000 plate from Porvair Sciences Ltd. maximises reflectivity enabling even weakl...

Read More
Hydrophilic Vyon® Plastics Demonstrate Outstanding Performance for Drug Discovery Applications

Hydrophilic Vyon® Plastics Demonstrate Outstanding Performance for Drug Discovery Applications

Porvair Sciences reports on testing that demonstrates how its hydrophilic Vyon® porous plastics exhibit outstanding performance in drug discovery appl...

Read More
Robot Compatible Solvent Removal Workstation

Robot Compatible Solvent Removal Workstation

The new UltraVap Mistral XT 150 from Porvair Sciences provides unmatched automated dry down of organic solvent-based samples in tubes and microplates ...

Read More

Read More

Read More

List your business

Would you like to register your company on one of the UK's largest and most effective Trade websites?

Are you looking for a reliable and consistent source of enquiries from your industry sector? Would you like to see why Find the Needle clients come back year after year to use our service? If so then feel free to register using the link 'here'.

Register
Office Address: